Protein preparation

This course contains five sub-courses, which consist of theoretical and practical parts. The individual sub-courses should provide students simple and quick approaches needed for solving basic scientific problems that could rise during the course of their bachelor, diploma or PhD study. The first set will allow students to gain knowledge about protein cloning, expression and purification. The second part focuses on biochemical characterization of enzymes via their enzymatic activity as well as protein-protein interactions. The third set will provide simple biochemical methods to detect protein-protein and protein-DNA interactions as well as monitor motor activity of protein.

The educational material is aimed to students of bachelor, master and doctoral study programmes.

Attachment   Date Size Availability [?] Clinically sensitive [?] Licence
 Pull down 19.6.2012 185.89 KB MEFANET user Creative Commons License
 Yeast two-hybrid 19.6.2012 439.96 KB MEFANET user Creative Commons License
 In vitro sumoylation assay 19.6.2012 86.6 KB MEFANET user Creative Commons License
 EMSA 19.6.2012 166.15 KB MEFANET user Creative Commons License
 Helicase activity assay 19.6.2012 163.21 KB MEFANET user Creative Commons License

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Graduate
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Advanced Graduated
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Complex
   

Hodnocení

Please select achieved education degree and then evaluate the teaching material particularly in light of material suitability for self-learning.
Student – student of bachelor or master degree
Graduate – graduate of bachelor or master degree
PhD. Graduate – Ph.D. student, Ph.D. graduate, researcher, ...
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