Multimedia support in the education of clinical and health care disciplines
MEFANET Project (MEdical FAculties Educational NETwork)
Faculty of Medicine, Masaryk University
Committee for Information Technologies in Medicine, MU’s Faculty of Medicine
Institute of Biostatistics and Analyses, Faculty of Medicine and Faculty of Science, Masaryk University

Database information on the 1st author of the contribution:
Lumír Krejčí
lkrejci(at)chemi.muni.cz
Department of Biology - Theoretical Departments - Faculty of Medicine
Faculty of Medicine
phone: +420 549 49 3767
fax: +420
address: Kamenice 5
625 00 Brno

Protein preparation

Protein preparation

This course contains five sub-courses, which consist of theoretical and practical parts. The individual sub-courses should provide students simple and quick approaches needed for solving basic scientific problems that could rise during the course of their bachelor, diploma or PhD study. The first set will allow students to gain knowledge about protein cloning, expression and purification. The second part focuses on biochemical characterization of enzymes via their enzymatic activity as well as protein-protein interactions. The third set will provide simple biochemical methods to detect protein-protein and protein-DNA interactions as well as monitor motor activity of protein.


The educational material is aimed to students of bachelor, master and doctoral study programmes.

Attachments:
Attachment   Date Size Availability [?] Clinically sensitive [?] Licence
pdf Pull down Download - Pull downOpen in new window - Pull down 19.6.2012 185.89 KB MEFANET user Creative Commons License
pdf Yeast two-hybrid Download - Yeast two-hybridOpen in new window - Yeast two-hybrid 19.6.2012 439.96 KB MEFANET user Creative Commons License
pdf In vitro sumoylation assay Download - In vitro sumoylation assayOpen in new window - In vitro sumoylation assay 19.6.2012 86.6 KB MEFANET user Creative Commons License
pdf EMSA Download - EMSAOpen in new window - EMSA 19.6.2012 166.15 KB MEFANET user Creative Commons License
pdf Helicase activity assay Download - Helicase activity assayOpen in new window - Helicase activity assay 19.6.2012 163.21 KB MEFANET user Creative Commons License
 

Keywords: Protein-protein and protein-DNA interaction methods, EMSA, pull-down, yeast 2 hybrid, Helicase assay, sumoylation

4-D assessment:

typ
Textbooks and manuals
typ
Educational websites and atlases
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Digital video
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Presentations and animations
typ
Casuistics in images
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E-learning courses (LMS)
result
Reviewed
information

Guarantee

Daniel Schwarz, PhD.
ICT, IBA MU
phone: +420 549 497866
e-mail: redakce.portal(at)med.muni.cz

Reviewers

doc. RNDr. Michal Otyepka, Ph.D
Katedra fyzikální chemie, Univerzita Palackého v Olomouci
phone: 58 563 4764
e-mail: michal.otyepka(at)upol.cz

doc. RNDr. Ladislav Dušek, Ph.D.
IBA, Masarykova univerzita
phone: 420549494469
e-mail: dusek(at)iba.muni.cz


level
Undergraduate level
level
Graduate
level
Advanced Graduated
level
Complex
   
Please select achieved education degree and then evaluate the teaching material particularly in light of material suitability for self-learning.
Student – student of bachelor or master degree
Graduate – graduate of bachelor or master degree
PhD. Graduate – Ph.D. student, Ph.D. graduate, researcher, ...
 
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this contribution first!
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Creative Commons LicenseContribution content is subject to licence Creative Commons Uveďte autora-Neužívejte dílo komerčně-Nezasahujte do díla Attribution 3.0 Czech Republic
author: Lumír Krejčí | organization: Department of Biology | published on: 18.6.2012 | last modified on: 20.8.2013
citation: Krejčí Lumír: Protein preparation. Multimedia support in the education of clinical and health care disciplines :: Portal of MU’s Faculty of Medicine [online] 2012-06-18, last modif. 2013-08-20 [cit. 2018-06-20] Available from WWW: <http://portal.med.muni.cz/article-593-protein-preparation.html>. ISSN 1801-6103.
 

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