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The study material “New methods in primary prevention of dental caries in children” is based on the need to present updated literature on primary prevention of dental caries in children, which reflects current trends and the latest findings of basic, applied and clinical research in the field of etiopathogenesis and prevention of dental caries. Due to the intensive scientific work of our team in this field, supported by a number of Czech and international publications, we have implemented results from molecular genetics and microbiology or behavioral intervention into the scripts. We point out the necessity of interdisciplinary cooperation and the benefits and interconnection of theoretical and clinical disciplines at medical and science faculties, not only in the framework of oral health research.