This course contains five sub-courses, which consist of theoretical and practical parts. The individual sub-courses should provide students simple and quick approaches needed for solving basic scientific problems that could rise during the course of their bachelor, diploma or PhD study. The first set will allow students to gain knowledge about protein cloning, expression and purification. The second part focuses on biochemical characterization of enzymes via their enzymatic activity as well as protein-protein interactions. The third set will provide simple biochemical methods to detect protein-protein and protein-DNA interactions as well as monitor motor activity of protein.
..keywords: ligation, transformation, plasmid preparation, protein purification, chromatography, sumoylation,.. ..keywords: transformation, plasmid preparation, protein purification, chromatography, sumoylation,..
Neural development and embryonic stem cell-based developmental modelling
.. techniques (e.g. gene cloning, making fusion protein, PCR and sequencing), and how to grow and to.. ..keywords: cloning, fusion protein, PCR, cDNA, cloning vectors, sequencing, human..
Analysis of genomic and proteomic data
.. from pre-processing through differential gene(protein)-expression to pathways and meta-analysis of..