Lumír Krejčí, lkrejci(at)chemi.muni.cz
The terms "recombinant DNA technology", "DNA cloning", "molecular cloning", and "gene cloning" all refer to the same process: a transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid. The DNA of interest can then be replicated (together with the rest of the plasmid) in a foreign host cell. This technology is a common practice in molecular biology labs today.
This course contains five sub-courses, which consist of theoretical and practical parts. The individual sub-courses should provide students simple and quick approaches needed for solving basic scientific problems that could rise during the course of their bachelor, diploma or PhD study. The first set will allow students to gain knowledge about protein cloning, expression and purification. The second part focuses on biochemical characterization of enzymes via their enzymatic activity as well as protein-protein interactions. The third set will provide simple biochemical methods to detect protein-protein and protein-DNA interactions as well as monitor motor activity of protein.